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主营:分子类,蛋白类,抗体类,生化类试剂
℡ 4000-520-616
℡ 4000-520-616
Innoprot/FUS/TLS Stress Granules Assay Cell Line/P30716
产品编号:P30716
市  场 价:¥199.90
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$10.00
品      牌: Innoprot
公      司:Innoprot
公司分类:
Innoprot/FUS/TLS Stress Granules Assay Cell Line/P30716
商品介绍

Amyotrophic lateral sclerosis (ALS) is one of the most common degenerative diseases of the motor neuron system. FUS/TLS and TDP-43, two strikingly similar RNA Binding Proteins, are dominant causes of both the fatal adult motor neuron disease amyotrophic lateral sclerosis (ALS) and frontal temporal degeneration (FTD). Stress granules (SGs) are granules of RNA and proteins formed in the cytosol of the cell under stressful conditions. Disease-linked mutations of FUS increase its propensity to aggregate and to form SGs by preventing nuclear translocation. FUS/TLS Stress Granules Assay Cell Line from Innoprot allows the identification of pathological stress granules formation inhibitors.

In normal conditions, FUS/TLS protein is predominantly localized in the nucleus. In the presence of an oxidative insult like Sodium arsenite, FUS/TLS increases its cytoplasmic localization forming Stress granules. This cell line allows the measurement of localization patterns to identify most promising test compounds.

To perform the assay, we incubate the cells with test compounds and Sodium Arsenite to induce the FUS/TLS aggregation. Finally, we quantify FUS/TLS-tGFP nuclear globs using the BD Pathway HCS Reader and Attovision Compartimentalization Software.

Applications:

  • FUS/TLS Stress Granules Assay cell line allows the quantification of pathological FUS/TLS Stress Granules.
  • This model permits to evaluate the FUS/TLS protein distribution in living cells studying the protein localization pattern in the space and time.
  • This model provides a strategy to evaluate drugs without cell permeability.
  • Reference: P30716
  • Size/Quantity: 2 vials containing 3x106 cells / vial
  • Product Use: For research use only
  • Shipping Conditions: Dry Ice
    • Data Sheet
    • Cell Culture Manual
    • Technical Poster
    品牌介绍

    Innoprot 为制药公司在研发和生产过程涉及到的DNA 重组技术, RNA 和蛋白相互作用等提供相关的各种原代细胞细胞系和细胞分析试剂盒,同时公司提供相关的检测定制服务、高通量分析检测、药物靶点优化服务。

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