The pancreas is an endocrine gland and digestive organ which secretes hormones and produces pancreatic juice to aid in digestion. Pancreatic endocrine cells, aka pancreatic islets, produce vital hormones such as glucagon, insulin, amylin, and somatostatin. In order to support the islets, the pancreas is a highly vascularized organ where endothelial cells play a critical role. Human Pancreatic microvascular endothelial cells (HPanMEC) not only function to transport oxygen and nutrients to the pancreas, but also affect beta cell function and proliferation, influence insulin gene expression during islet development, and produce various growth factors associated with vasoactivity. Additionally, PanMEC participate in optimizing blood glucose sensing and regulation. PanMEC play an important role in the development and progression of pancreatic cancer. Human PaMEC are a useful in vitro model for studying islet biology, pancreatic cancer, transplantation, and regenerative medicine.