Adipocyte Medium (AdM) is developed as a maintenance medium used after differentiation of human preadipocytes to mature adipocytes in vitro. It is a sterile liquid medium, which contains all of the components necessary to support lipid accumulation in the maturing adipocytes. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air.