Our Preadipocyte Differentation Medium (PADM) has been specifically developed and optimized for in vitro differentiation of preadipocytes into mature adipocytes. PADM is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air